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Human spleen histone H4. Isolation and amino acid sequence
Journal of Biochemistry
|December 1, 1982
Summary
Human histone H4 amino acid sequence is identical to calf thymus H4, revealing strong evolutionary conservation. This suggests histone H4 sequences and modifications are highly preserved across species.
Area of Science:
- Biochemistry
- Molecular Biology
- Evolutionary Biology
Background:
- Previous studies analyzed human spleen histones H2B, H2A, and H3.
- Histone evolution research requires detailed sequence analysis.
Purpose of the Study:
- Determine the complete amino acid sequence of human spleen histone H4.
- Investigate the evolutionary conservation of histone H4.
Main Methods:
- Purification of histone H4 using Bio-Gel P-60 chromatography.
- Trypsin digestion and peptide fractionation.
- Amino acid sequencing of purified peptides.
Main Results:
- Identified three modified residues: alpha-N-Ac-Ser-1, epsilon-N-Ac-Lys-16, and epsilon-N-Me-Lys-20.
- Found a heterogeneous residue at position 25 (Asn/Asp), likely due to deamidation.
- Deduced a 102-amino acid sequence identical to calf thymus H4.
Conclusions:
- Human and calf thymus histone H4 sequences are identical.
- Histone H4 sequences and postsynthetic modifications show strong evolutionary conservation.
- Histone H4 conservation exceeds that of H3, H2A, and H2B.