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KCN-induced urinary chemiluminescence (UCL): inhibition by ascorbic acid
Research Communications in Chemical Pathology and Pharmacology
|November 1, 1982
Summary
Chemiluminescence in human urine is triggered by potassium cyanide (KCN) and hydrogen peroxide (H2O2). These reactions involve distinct urine compounds, offering insights into urine analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Chemiluminescence (CL) detection offers a sensitive analytical method.
- Urine contains various compounds that may interact with oxidants and other chemicals.
Purpose of the Study:
- To investigate the chemiluminescence produced by adding potassium cyanide (KCN) and hydrogen peroxide (H2O2) to normal human urine.
- To characterize the chemical nature of the urine components responsible for these CL reactions.
Main Methods:
- Addition of KCN and H2O2 to human urine samples.
- Varying concentrations of KCN, H2O2, and pH.
- Assessing inhibition by various compounds including ascorbic acid (AA) and hydroxyl radical scavengers.
- Sephadex G15 gel filtration chromatography.
Main Results:
- Both KCN and H2O2 induced CL in urine, increasing with concentration and pH.
- KCN-induced CL was inhibited by AA, glucose, albumin, tryptophan, hemoglobin, and histidine.
- H2O2-induced CL was primarily inhibited by tryptophan.
- Hydroxyl radical scavengers showed minimal effect.
- Chromatography revealed distinct KCN- and H2O2-sensitive components with molecular weights <1500 daltons.
Conclusions:
- The chemiluminescence generated by KCN and H2O2 in urine originates from interactions with chemically distinct compounds.
- These findings suggest potential for specific analytical assays based on urine chemiluminescence.