Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calibrant solutions for continuous-flow urate analysis: interference by formaldehyde

R Beetham, P G Sanders

    Annals of Clinical Biochemistry
    |November 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Whiplash injuries of the cervical spine.

    The Australian journal of physiotherapy·2014
    Same author

    The role of alpha1-antitrypsin in total hip replacement: a pilot study.

    Hip international : the journal of clinical and experimental research on hip pathology and therapy·2009
    Same author

    Effects of storage at -20 degrees C on ischaemia-modified albumin results.

    Annals of clinical biochemistry·2006
    Same author

    The PRIMA study: presentation ischaemia-modified albumin in the emergency department.

    Emergency medicine journal : EMJ·2006
    Same author

    Cerebrospinal fluid spectrophotometry of bilirubin, not the Xanthochromic Index, for the detection of CT-negative sub-arachnoid haemorrhage.

    Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2006
    Same author

    Is cerebrospinal fluid spectrophotometry useful in CT scan-negative suspected subarachnoid haemorrage?

    European neurology·2004
    Same journal

    Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

    Annals of clinical biochemistry·2026
    Same journal

    From interpretable NT-proBNP prediction to equitable laboratory stewardship.

    Annals of clinical biochemistry·2026
    Same journal

    Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

    Annals of clinical biochemistry·2026
    Same journal

    Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

    Annals of clinical biochemistry·2026
    Same journal

    Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

    Annals of clinical biochemistry·2026
    Same journal

    Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

    Annals of clinical biochemistry·2026
    See all related articles

    Formaldehyde in urate calibration solutions interferes with AutoAnalyzer systems by affecting dialysis rates below pH 8.5. However, purely aqueous urate calibrants are suitable for these systems, despite formaldehyde interference.

    Area of Science:

    • Clinical chemistry
    • Analytical chemistry
    • Biochemistry

    Background:

    • Formaldehyde has been used since 1913 to preserve aqueous urate calibration solutions.
    • Formaldehyde can interfere with certain analytical methods, particularly in automated systems.
    • Understanding this interference is crucial for accurate clinical diagnostics.

    Purpose of the Study:

    • To investigate the interference of formaldehyde in urate calibration solutions with Technicon AutoAnalyzer systems.
    • To determine the effect of pH on formaldehyde interference with urate dialysis.
    • To identify the chemical basis of the interference and assess alternative calibrant solutions.

    Main Methods:

    • Studying urate dialysis rates at varying pH levels in the presence of formaldehyde.

    Related Experiment Videos

  • Utilizing thin-layer chromatography and mass spectrometry to analyze formaldehyde-uric acid interactions.
  • Conducting recovery experiments with protein-containing solutions.

    • Main Results:

    • Formaldehyde interferes with urate dialysis in AutoAnalyzer systems at pH below 8.5.
    • The interference is due to the formation of pH-dependent addition products between uric acid and formaldehyde.
    • Purely aqueous urate calibrant solutions demonstrated satisfactory performance in AutoAnalyzer systems.

    Conclusions:

    • The interference of formaldehyde in urate calibration is pH-dependent and linked to specific chemical interactions.
    • Purely aqueous urate calibrants can be reliably used in AutoAnalyzer systems, circumventing formaldehyde-related interferences.
    • This finding supports the use of alternative calibration strategies for accurate urate measurements.