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Human bone marrow contains fibroblast colony forming cells
The Tokai Journal of Experimental and Clinical Medicine
|October 1, 1980
Summary
Human bone marrow fibroblast colonies develop in liquid culture within 4 days, stimulated by fetal calf serum. This method aids hematological disorder studies.
Area of Science:
- Hematology
- Cell Biology
- Tissue Engineering
Background:
- Fibroblast colonies are crucial in bone marrow microenvironments.
- Understanding fibroblast development is key for studying hematological disorders.
Purpose of the Study:
- To establish and characterize a liquid culture method for human bone marrow fibroblast colony development.
- To assess the utility of this method for hematological research.
Main Methods:
- Human bone marrow cells were cultured in liquid medium.
- Fetal calf serum (20%) was used to stimulate fibroblast colony growth.
- Colony formation was quantified per nucleated cell.
Main Results:
- Fibroblast colony development initiated on day 4 of liquid culture.
- Human bone marrow yielded 47 +/- 4 fibroblast colonies per 2 X 10(5) nucleated cells.
- Agar or methylcellulose-based cultures restricted colony formation.
Conclusions:
- Liquid culture provides a viable method for human bone marrow fibroblast colony formation.
- This technique shows promise for pathognomonic and clinical investigations in hematological disorders.