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Related Experiment Videos

Human blood preservation: effect on in vitro protein binding

A J Jackson, A K Miller, P K Narang

    Journal of Pharmaceutical Sciences
    |October 1, 1981
    PubMed
    Summary

    The type of preserved human blood significantly impacts in vitro drug binding. Fresh heparinized plasma and serum yielded consistent results, unlike citrated plasma or lyophilized serum for certain drugs.

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    Area of Science:

    • Pharmacology
    • Clinical Chemistry
    • Biochemistry

    Background:

    • In vitro drug binding assays are crucial for pharmacokinetic studies.
    • Variations in sample matrices can influence drug-protein interactions.
    • Understanding these variations is essential for accurate drug concentration interpretation.

    Purpose of the Study:

    • To investigate the effect of different human blood preservation methods on in vitro plasma protein binding of phenytoin, meperidine, and bretylium tosylate.
    • To compare drug binding in fresh heparinized plasma/serum versus fresh citrated plasma and lyophilized human serum.
    • To determine the extent of variability introduced by sample matrix differences.

    Main Methods:

    • In vitro assessment of plasma protein binding for three drugs: phenytoin, meperidine, and bretylium tosylate.
    • Utilized fresh heparinized plasma, fresh citrated plasma, and commercially available lyophilized human serum as matrices.
    • Drug concentrations were maintained at studied levels for all estimations.

    Main Results:

    • Fresh heparinized plasma and serum showed equivalent binding fractions for all drugs.
    • Phenytoin and meperidine binding decreased by 9-50% in fresh citrated plasma and lyophilized serum.
    • Bretylium tosylate binding decreased by 30-40% in fresh citrated plasma but remained unchanged in lyophilized serum.

    Conclusions:

    • The choice of preserved human blood for in vitro drug binding assays can significantly alter results.
    • Citrated plasma and lyophilized serum may lead to underestimation of plasma protein binding for certain drugs.
    • Careful consideration of sample matrix is necessary for reliable in vitro drug binding studies.

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