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Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel
The Biochemical Journal
|January 1, 1981
Abstract:
Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.