[3-oxosteroid-delta 1-dehydrogenase localization in Mycobacterium rubrum and Arthobacter globiformis cells]

Osmotically susceptible forms were obtained from Mycobacterium rubrum and Arthrobacter globiformis (Mycobacterium globiforme) cells. The activity of 3-oxosteroid-delta 1-dehydrogenase was comparatively estimated in subcellular fractions after differential centrifugation of cell homogenates prepared either mechanically or by lysis of osmotically susceptible forms. The results indicate that the enzyme is located in the cells of the above microorganisms in both the free state (in the fraction of soluble proteins) and the membrane-bound form.