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![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
[Coincidence of the process of hydrogen-oxidizing bacterial culture and nutrient medium electrolysis]
Abstract:
The main factors limiting the possibility of organizing the cultivation of the hydrogen-oxidizing bacterium Alcaligenes eutropha Z-1 in combination with electrolysis were determined: accumulation of the oxidizing agents (hypochlorite, persulfate ions, hydrogen peroxide) in the process of electrolysis and deposition of microelements contained in the growth medium on the cathode. The limits for the susceptibility of the bacterium to the oxidizing agents were established. The rate of accumulation of the oxidizing agents and formation of the cathode deposit were found to depend on the current density, the pH and composition of a solution, and several other factors. The concentration of hypochlorite decreased if the content of chloride ions in the growth medium was reduced to approximately 10(-5) g/l; the concentrations of persulfate and hydrogen peroxide is decreased using a certain electrolysis regime and adding ferrous ions which reduce the oxidizing agents. The cathode deposit is dissolved by periodically reversing the current in the course of electrolysis.
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