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[Typical nature of th primary short-term bacteremia in experimental shigellosis]
Abstract:
The comparative histological study of morphological changes in the intestine of monkeys and in the isolated loop of the small intestine of rabbits and mice infected with dysentery bacteria showed the possibility of using the intragastric infection of white mice as a model allowing one to study some aspects of the pathogenesis of dysentery. In experiments on monkeys, rabbits and white mice infected with Sh. dysenteriae, Sh, flexneri, Sh, newcastle, Sh, boydii and Sh. sonnei the presence of shortterm bacteremia was revealed in all cases of infection. The systematic detection of the causative agents of dysentery in the blood was possible only during the first 6 hours of the experimental infectious process. The release of shigellae from the intestine into the blood flow and their dissemination was confirmed by the detection of shigellae in the blood and internal organs of the animals in the bacteriological study and by the fluorescent antibody method.
Insights
This study demonstrates that intragastric infection in mice effectively models dysentery pathogenesis. Shigella bacteria were detected in the bloodstream early in infection across multiple animal models, confirming bacteremia.
Area of Science:
- Microbiology
- Pathogenesis
- Histology
Context:
- Dysentery pathogenesis research often relies on animal models.
- Understanding bacterial dissemination is crucial for disease management.
Purpose:
- To compare intestinal morphological changes in monkeys, rabbits, and mice infected with Shigella.
- To validate intragastric infection in mice as a model for studying dysentery.
- To investigate the occurrence and timing of bacteremia during Shigella infection.
Summary:
- Histological examination of intestinal changes in monkeys, rabbits, and mice infected with various Shigella species (Sh. dysenteriae, Sh. flexneri, Sh. newcastle, Sh. boydii, Sh. sonnei) was conducted.
- Intragastric infection in white mice was identified as a viable model for studying aspects of dysentery pathogenesis.
- Short-term bacteremia was observed in all infected animals, with Shigella consistently detected in the blood and internal organs within the first 6 hours post-infection, confirmed by bacteriological and fluorescent antibody methods.
Impact:
- Establishes a reliable mouse model for dysentery research.
- Provides evidence for early-stage bacteremia in Shigella infections.
- Highlights the importance of early detection for understanding disease progression.