[Biological applications of Raman resonance spectroscopy and micro-Raman resonance spectroscopy]

Advantages are given to illustrate the possibilities of resonance Raman spectroscopy in the studies of biological systems. A relation giving the size of porphinato-core of metalloporphyrins from resonance Raman frequency is proposed. It can be applied to heme, even in lived state. By illuminating the sample through a microscope, resonance Raman effect can be collected from 1 (micrometer)2 of sample. Spectra of a vegetal-cell wall (pimento) and of a red corpuscle (hemoglobin) are shown as examples.