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Updated: Jul 19, 2026
15:04
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Microbial chromophore materials in circulating blood identified by laser micro Raman spectroscopy.
Biorheology. Supplement : the Official Journal of the International Society of Biorheology
|January 1, 1984
Summary
Blue particles found in pathological blood, including cancer and leukemia, are linked to bacteria. These microbes can produce the colored particles in vitro, suggesting a microbial origin for these blood anomalies.
Area of Science:
- Microbiology
- Hematology
- Spectroscopy
Background:
- Microscopic examination of pathological blood (infectious diseases, cancer, leukemia) reveals micrometer-sized blue particles.
- These particles are observed in blood samples from patients with various serious illnesses.
Purpose of the Study:
- To investigate the origin of the blue, micrometer-sized particles observed in pathological blood.
- To determine if bacteria are responsible for producing these chromophore particles in blood.
Main Methods:
- Microbial cultivation of blood samples from pathological cases.
- Cultivation of isolated bacteria on Chocolate Blood Medium.
- Micro-Raman Spectrometry analysis of blue particles from both pathological blood and in vitro cultures.
- Comparison of vibrational spectra to establish particle identity.
Main Results:
- Bacteria were isolated from pathological blood samples, capable of producing blue chromophore particles in vitro.
- These bacteria induced the appearance of blue particles in normal blood.
- Micro-Raman Spectroscopy confirmed the identical nature of blue particles from pathological blood and bacterial cultures.
- The findings strongly suggest a bacterial origin for the blue particles in pathological blood.
Conclusions:
- The blue particles observed in pathological blood, particularly in cancer and leukemia cases, are likely produced by bacteria.
- This discovery opens new avenues for understanding pathogenic mechanisms and potential diagnostic markers.
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