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Retinol esterification in lamb kidney

S Donoghue, K Johnson, W J Donawick

    Journal of Dairy Science
    |December 1, 1981
    PubMed
    Summary
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    Lamb kidneys esterify vitamin A (retinol) in vivo and in vitro. This process, linked to fatty acyl coenzyme A:retinol acyl transferase, may help regulate vitamin A levels in the body.

    Area of Science:

    • Biochemistry
    • Animal Science
    • Physiology

    Background:

    • Vitamin A (retinol) is essential for numerous physiological processes.
    • Understanding retinol metabolism, particularly esterification, is crucial for maintaining vitamin A homeostasis.
    • The role of the kidney in vitamin A esterification has not been fully elucidated.

    Purpose of the Study:

    • To investigate the in vivo and in vitro esterification of retinol in lamb kidney.
    • To identify the cellular location and key factors involved in renal retinol esterification.
    • To explore the potential role of renal retinol esterification in vitamin A homeostasis.

    Main Methods:

    • In vivo studies involved injecting radiolabeled retinol into the renal artery of lambs and analyzing renal venous effluent.

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  • In vitro studies utilized kidney homogenates incubated with adenosine 5'-triphosphate and coenzyme A.
  • Enzyme activity was measured in different subcellular fractions, particularly microsomes.

    • Main Results:

    • Retinol esterification was confirmed in lamb kidneys, with labeled retinyl esters detected in the renal vein after arterial injection.
    • The degree of retinol esterification increased with higher vitamin A intake.
    • Enzyme activity was primarily localized in kidney microsomes and was significantly enhanced by adenosine 5'-triphosphate and coenzyme A.

    Conclusions:

    • Renal retinol esterification is likely catalyzed by fatty acyl coenzyme A:retinol acyl transferase.
    • Kidney microsomes are the primary site of this enzymatic activity.
    • Renal esterification may play a significant role in the body's overall vitamin A homeostasis.