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In vitro study of complement dependent active adherence on SLE and RA leukocytes
Acta Physiologica Academiae Scientiarum Hungaricae
|January 1, 1981
Summary
Leukocyte adherence in autoimmune diseases like SLE and RA is altered, showing decreased attachment in normal serum. Specific differences were observed between SLE and RA patients when comparing native and heat-inactivated sera, indicating complement involvement.
Area of Science:
- Immunology
- Cell Biology
- Autoimmune Diseases
Background:
- Immunological disorders can affect leukocyte function, including their adherence.
- Leukocyte adherence is crucial for immune responses and can be impaired in autoimmune conditions.
Purpose of the Study:
- To investigate the active adherence of leukocytes from healthy individuals and patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA).
- To determine the complement dependency of leukocyte adherence.
- To compare the effects of native and heat-inactivated human sera on leukocyte adherence in SLE and RA patients.
Main Methods:
- Studied active adherence of buffy coat cells on glass surfaces.
- Compared leukocyte adherence in serum-free medium versus normal human serum.
- Assessed effects of native and heat-inactivated human sera.
- Measured C3 levels in leukocyte supernatants using rocket electrophoresis.
- Detected surface-bound C3 on leukocytes via immunofluorescence.
Main Results:
- Leukocyte adherence was reduced in normal serum for both healthy individuals and patients with SLE and RA.
- SLE leukocytes showed higher adherence in unheated serum compared to heat-inactivated serum.
- RA leukocytes exhibited lower adherence in unheated serum versus heat-inactivated serum.
- C3 deposition was primarily observed on SLE leukocytes.
- Supernatants from patient leukocytes influenced control leukocytes similarly to their own disease state.
Conclusions:
- Leukocyte adherence is significantly altered in SLE and RA.
- Complement system, particularly C3, plays a role in the differential adherence patterns observed in SLE and RA.
- These findings highlight distinct immunological mechanisms in SLE and RA concerning leukocyte-endothelial interactions.