Related Experiment Videos
[Isolation and study of several properties of mycoplasma from Japanese quail embryos]
Voprosy Virusologii
|September 1, 1978
Abstract:
An agent isolated from Japanese quail embryos was found to be pathogenic for chick embryo and quail embryo fibroblast cultures, to cause hemadsorption and hemagglutination of guinea pig erythrocytes. Reproduction of the agent was inhibited by tetracycline and 5-bromodeoxyuridine. The agent incorporated both 3H-thymidine and 3H-uridine and had a buoyant density in sucrose gradient of 1.22 g/ml. Mature mycoplasma forms were determined electron microscopically in gradient fractions in cell sections.
Insights
A novel Japanese quail embryo agent is pathogenic to avian cells, causing hemadsorption and hemagglutination. This mycoplasma agent
Area of Science:
- Avian pathology
- Microbiology
- Virology
Context:
- Isolation of a novel infectious agent from Japanese quail embryos.
- Observation of pathogenic effects on avian cell cultures.
Purpose:
- Characterize the agent's pathogenicity and biological properties.
- Investigate the agent's replication and morphology.
Summary:
- The agent causes hemadsorption and hemagglutination in guinea pig erythrocytes.
- Agent replication is inhibited by tetracycline and 5-bromodeoxyuridine.
- The agent incorporates nucleic acid precursors and has a buoyant density of 1.22 g/ml, with mature mycoplasma forms identified electron microscopically.
Impact:
- Provides insights into avian pathogens and their mechanisms.
- Contributes to understanding mycoplasma biology.
- Potential implications for avian health and disease control.