[Isolation of hepatitis B (HBSAg) from donor blood plasma]

An original method for purification of HBsAg from the blood plasma of donors is described. Preparations have been obtained containing structures 22 nm in size the proteins of which form 9 bands in polyacrylamide gel electrophoresis and containing no detectable amounts of admixtures accompanying HBsAg. The purified HBsAg meets the requirements for test-antigens for radioimmuniassay and immune autoradiography. The reported method may be used for preparation of HBsAg under production conditions.