Plasma membrane associated proteins and glycoproteins of a temperature-sensitive hamster cell line (ts 3)

Hamster ts 3 cells grown at 32 and at 39 degrees C were compared for (a) cell phenotype and growth rates, (b) sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) patterns of plasma membrane polypeptides, and (c) distribution of radioactivity in SDS-PAGE of plasma membrane glycopeptides metabolically labeled with [3H]glucosamine and [3H]fucose. It was observed that ts 3 cells growing at 39 degrees C and having a normal phenotype contained increased amounts of a glycopeptide of about 250000 daltons and a polypeptide of about 200000 daltons, as compared with ts 3 cells grown at 32 degrees C and having a transformed phenotype. No other alterations of major significance were observed.