Trichostrongylus colubriformis: cultivation of free-living stages

Successful cultivation of Trichostrongylus colubriformis from hatched first-stage to third-stage larvae was achieved in media containing NCTC 135, chick embryo extract, fetal calf serum, and either lactalbumin hydrolysate or freshly prepared baker's yeast extract. Medium Tc5, containing yeast extract, was the simplest medium with optimal results. Cultured third-stage larvae were able to produce patent infections in guinea pigs.