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Binding of non-mammalian immunoglobulins to staphylococcal protein A
Folia Biologica
|January 1, 1980
Immunoglobulins M of the chicken (about 3% of serum IgM), clawed toad (12%) and carp (13%) bind to the protein A-Sepharose. A monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). The protein A-Sepharose can be utilized for isolation of IgM of the clawed toad and carp directly from the serum.
Immunoglobulins M of the chicken (about 3% of serum IgM), clawed toad (12%) and carp (13%) bind to the protein A-Sepharose. A monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). The protein A-Sepharose can be utilized for isolation of IgM of the clawed toad and carp directly from the serum.