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[Lysogenic conversion of cholera vibrios]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|July 1, 1980
Summary
Prophage elimination from Vibrio cholerae was achieved using various treatments. This elimination altered bacterial properties, with some changes linked to specific phage types influencing serology and sensitivity.
Area of Science:
- Microbiology
- Virology
- Genetics
Background:
- Lysogenic Vibrio cholerae strains, biotype El Tor, harbor prophages.
- Prophages can influence bacterial characteristics and host-pathogen interactions.
- Understanding prophage-bacteria relationships is crucial for cholera research.
Purpose of the Study:
- To eliminate prophages from wild lysogenic Vibrio cholerae strains.
- To investigate the impact of prophage elimination on bacterial properties.
- To identify specific phage races involved in bacterial trait conversion.
Main Methods:
- Induction of prophage elimination using ultraviolet irradiation.
- Chemical treatments including nitrosomethyl urea, mitomycin C, ascorbic acid, and sarcolysin.
- Analysis of biological property changes in Vibrio cholerae post-prophage elimination.
Main Results:
- Successful elimination of prophage from Vibrio cholerae El Tor strains was achieved.
- Prophage elimination resulted in alterations of bacterial biological properties.
- Specific type II phage races were identified as responsible for converting serological characteristics, diagnostic phage sensitivity, and polymyxin resistance.
Conclusions:
- Prophage elimination is feasible in Vibrio cholerae using various agents.
- Bacterial properties are significantly influenced by prophage carriage.
- Certain temperate phages play a critical role in determining Vibrio cholerae's phenotype, including serotype and antimicrobial sensitivity.