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Effect of platelets on apparent leucocyte ascorbic acid content

R M Evans, L Currie, A Campbell

    Annals of Clinical Biochemistry
    |September 1, 1980
    PubMed
    Summary
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    Leukocyte ascorbic acid measurements can be overestimated due to platelet contamination. This study shows a significant correlation between platelet levels and apparent leukocyte ascorbic acid, impacting accurate tissue status assessment.

    Area of Science:

    • Biochemistry
    • Hematology
    • Nutrition Science

    Background:

    • Leukocyte ascorbic acid content is a common indicator of tissue vitamin C status.
    • Current analysis methods often include platelets in the buffy layer, leading to overestimation.
    • Platelet ascorbic acid contribution can skew the interpretation of leukocyte vitamin C levels.

    Purpose of the Study:

    • To investigate the impact of platelet contamination on leukocyte ascorbic acid measurements.
    • To determine the correlation between platelet:leukocyte ratio and measured leukocyte ascorbic acid.
    • To highlight potential inaccuracies in assessing tissue vitamin C status using standard methods.

    Main Methods:

    • Analysis of blood samples from healthy individuals on ascorbic acid supplements and patients on high-dose therapy.

    Related Experiment Videos

  • Measurement of ascorbic acid content in the buffy layer (leukocytes and platelets).
  • Statistical analysis to correlate 'leukocyte' ascorbic acid content with the platelet:leukocyte ratio.

    • Main Results:

    • A significant positive correlation (r = 0.70, P < 0.001) was found between 'leukocyte' ascorbic acid content and the platelet:leukocyte ratio.
    • This indicates that variations in platelet and leukocyte distribution affect measured ascorbic acid levels.
    • Ascorbic acid levels in buffy layer samples are influenced by platelet concentration.

    Conclusions:

    • Standard methods for measuring leukocyte ascorbic acid may overestimate vitamin C status due to platelet interference.
    • Changes in platelet:leukocyte ratios can create apparent shifts in ascorbic acid levels.
    • Re-evaluation of analytical methods is needed for accurate assessment of tissue vitamin C status.