Related Experiment Video
Updated: Oct 1, 2026

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Immunoelectgrophoretic pattern of Klebsiella O and K antigens
Abstract:
Klebsiella capsular antigens K1-K72 and O antigens O1-O9 were isolated and rabbits immunized with corresponding bacteria. The immunoelectrophoretic patterns of the O and K antigens were studied. All K antigens analyzed were highly electrophoretically mobile, giving a marked precipitate of different lengths towards the anode. All the O antigens analyzed with the exception of O4 were nonmobile and gave a precipitate next to the start basin. The O4 precipitated towards the anode. These data indicate a possibility for using counterimmunoelectrophoresis in the diagnosis of Klebsiella antigen in body fluids or for capsular typing of the bacteria using the same technique.
More Related Videos
12:49Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
Published on: September 4, 2011
12:18An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
Related Concept Videos
Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunogold Electron Microscopy
Electrophoresis: Overview
There...