Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The evolution of WT1 sequence and expression pattern in the vertebrates

J Kent1, A M Coriat, P T Sharpe

  • 1MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.

Oncogene
|November 2, 1995
PubMed
Summary

The WT1 gene is crucial for kidney and gonad development. Its key regulatory domains, including zinc fingers, are highly conserved across vertebrate species, indicating ancient functional importance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Involvement of X-Inactivation in Organogenesis.

Journal of dental research·2026
Same author

Computational Methods for Image Analysis in Craniofacial Development and Disease.

Journal of dental research·2024
Same author

Redefining Mucosal Inflammation with Spatial Genomics.

Journal of dental research·2024
Same author

GSK3 Inhibitor-Induced Dentinogenesis Using a Hydrogel.

Journal of dental research·2021
Same author

Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration.

Materials today. Bio·2021
Same author

Translation Approach for Dentine Regeneration Using GSK-3 Antagonists.

Journal of dental research·2020

Area of Science:

  • Developmental Biology
  • Evolutionary Biology
  • Genetics

Background:

  • The WT1 gene is a critical regulator of kidney and gonadal development.
  • WT1 mutations are linked to Wilms' tumour, a kidney cancer.
  • Understanding WT1's evolutionary conservation can elucidate genitourinary system development.

Purpose of the Study:

  • To investigate the evolutionary conservation of WT1 gene orthologues across gnathostome classes.
  • To identify key regulatory domains of WT1 involved in vertebrate genitourinary system development and evolution.

Main Methods:

  • Isolation of WT1 orthologues from various vertebrate species (chick, alligator, Xenopus laevis, zebrafish).
  • Partial nucleotide sequencing and comparative analysis of conserved domains.

Related Experiment Videos

  • Examination of alternative splicing regions and their presence across species.

    • Main Results:

    • High sequence similarity observed in WT1 zinc fingers and transregulatory domains across all examined species.
    • The KTS insertion (3 amino acids) between zinc fingers 3 and 4 is conserved in non-mammalian species.
    • A C-terminal 17 amino acid insertion is unique to mammals.
    • Conserved expression patterns in intermediate mesoderm of chick and alligator, similar to mammals.
    • WT1 expression also noted in other mesodermal derivatives, suggesting a broader role.

    Conclusions:

    • WT1's core regulatory domains are highly conserved throughout vertebrate evolution.
    • Alternative splicing patterns of WT1 differ between mammals and other vertebrates.
    • WT1 plays a conserved role in genitourinary development and potentially broader mesodermal differentiation across vertebrates.