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Updated: Aug 14, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
Published on: November 26, 2014
[Emerson-Trinder reaction: study of various chromogens and analyses of the principle interferences (author's transl)]
Abstract:
Several phenolic compounds and aromatic amines were tested with a system consisting of hydrogen peroxide, peroxidase and 4-aminophenazone for their chromogenic activity in the Emerson-Trinder reaction: sulphonated 2,4-diclorophenol had the greatest activity, about 4 times that of unsubstituted phenol. The mechanism of action of serum components (serum proteins, bilirubin and ascorbic acid) which can interfere in the Emerson-Trinder reaction was also investigated by the same analytical system. Binding of phenolic compounds to serum was found to be responsible for protein interference: this can be overcome by increasing the phenolic compounds concentration. The interference by bilirubin and ascorbic acid is due to hydrogen peroxide consumption, in competition with the chromogenic substrate.
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