Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA editing in trypanosomes

R Benne1

  • 1E.C. Slater Institute, University of Amsterdam, The Netherlands.

European Journal of Biochemistry
|April 1, 1994
PubMed
Summary
This summary is machine-generated.

RNA editing in trypanosomes involves uridylate insertion/deletion in mitochondrial pre-mRNAs. Guide RNAs provide editing information, crucial for generating translatable messenger RNAs (mRNAs) in African trypanosomes like Trypanosoma brucei.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

+1 Proteins and aging.

The international journal of biochemistry & cell biology·2002
Same author

Cloning and characterization of two guide RNA-binding proteins from mitochondria of Crithidia fasciculata: gBP27, a novel protein, and gBP29, the orthologue of Trypanosoma brucei gBP21.

Nucleic acids research·2001
Same author

Molecular misreading: a new type of transcript mutation expressed during aging.

Neurobiology of aging·2000
Same author

Molecular misreading. A new type of transcript mutation in gerontology.

Annals of the New York Academy of Sciences·2000
Same author

Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks.

RNA (New York, N.Y.)·2000
Same author

MitBASE : a comprehensive and integrated mitochondrial DNA database. The present status.

Nucleic acids research·1999
Same journal

Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

European journal of biochemistry·2020
Same journal

AB 3.1.1.1 (or EC 3.1.1.?).

European journal of biochemistry·2020
Same journal

Cdk5.

European journal of biochemistry·2018
Same journal

Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

European journal of biochemistry·2004
Same journal

Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

European journal of biochemistry·2004
Same journal

Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

European journal of biochemistry·2004
See all related articles

Area of Science:

  • Molecular Biology
  • Genetics
  • Parasitology

Background:

  • Mitochondrial pre-mRNAs in trypanosomes undergo posttranscriptional editing.
  • This editing involves uridylate (U) insertion and deletion.
  • Massive editing shapes over 50% of coding sequences in some African trypanosome mRNAs.

Purpose of the Study:

  • To review research on trypanosome RNA editing.
  • To illustrate differing interpretations of editing mechanisms and biochemistry.
  • To highlight the role of guide RNAs in gene expression.

Main Methods:

  • Review of existing research on RNA editing in trypanosomes.
  • Analysis of partially edited RNAs and chimeric molecules.
  • In vivo and in vitro studies of guide RNA-mRNA interactions.

Related Experiment Videos

Main Results:

  • RNA editing is essential for generating translatable mRNAs in trypanosomes.
  • Guide RNAs, encoded in mitochondrial DNA, provide editing information.
  • Chimeric molecules suggest a covalent linkage between guide RNAs and pre-mRNAs.

Conclusions:

  • RNA editing is a critical step in trypanosome gene expression.
  • Understanding editing mechanisms requires reconciling diverse experimental data.
  • Further research is needed to elucidate the precise biochemistry of RNA editing.