Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse transcriptase inhibition as prescreen for potential antiviral bioactives

M D Antoun1, Y R Ríos, N T Mendoza

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, University of Puerto Rico, San Juan 00936-5067.

Puerto Rico Health Sciences Journal
|March 1, 1994
PubMed
Summary

Plant extracts were tested for their ability to inhibit avian myeloblastosis virus reverse transcriptase. This screening method can help identify potential compounds for developing anti-HIV drugs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, Toronto, Ont., September 6-9, 2007.

Canadian journal of surgery. Journal canadien de chirurgie·2023
Same author

Repeated temporary threshold shift and changes in cochlear and neural function.

Hearing research·2019
Same author

[Medicaments and oral healthcare. Systematic review of the -literature assessing the effect of drugs on the salivary glands].

Nederlands tijdschrift voor tandheelkunde·2018
Same author

World Workshop on Oral Medicine VI: a systematic review of medication-induced salivary gland dysfunction.

Oral diseases·2015
Same author

Composition of enamel pellicle from dental erosion patients.

Caries research·2014
Same author

Comparison of the possible protective effect of the salivary pellicle of individuals with and without erosion.

Caries research·2013

Area of Science:

  • Phytochemistry
  • Molecular Biology
  • Virology

Background:

  • Reverse transcriptase (RT) is a crucial enzyme in retroviral replication.
  • Avian myeloblastosis virus (AMV) reverse transcriptase is a model enzyme for studying RT activity.
  • Natural products, particularly plant extracts, are a rich source of bioactive compounds.

Purpose of the Study:

  • To evaluate the inhibitory potential of higher plant extracts against AMV reverse transcriptase.
  • To establish a prescreening assay for identifying novel anti-HIV bioactive compounds from natural sources.

Main Methods:

  • Preparation of various plant extracts.
  • Assay of AMV reverse transcriptase activity in the presence of plant extracts.
  • Quantification of enzyme inhibition based on RT activity.

Related Experiment Videos

Main Results:

  • Several plant extracts demonstrated significant inhibitory activity against AMV reverse transcriptase.
  • The developed assay effectively identified potential inhibitors.

Conclusions:

  • Higher plant extracts contain compounds that can inhibit avian myeloblastosis virus reverse transcriptase.
  • This assay serves as a valuable prescreen for discovering anti-HIV therapeutic agents from botanical sources.