Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bivariate flow karyotyping with air-cooled lasers

T Frey1, D W Houck, B J Shenker

  • 1Becton Dickinson Immunocytometry Systems, San Jose, California 95131.

Cytometry
|June 1, 1994
PubMed
Summary

An experimental flow cytometer achieved comparable resolution to standard instruments for analyzing human chromosomes. This new system demonstrates effective multilaser excitation for bivariate flow karyotyping.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A High-Resolution Tile-Based Approach for Classifying Biological Regions in Whole-Slide Histopathological Images.

IFMBE proceedings·2016
Same author

Variants of Porphyromonas gingivalis lipopolysaccharide alter lipidation of autophagic protein, microtubule-associated protein 1 light chain 3, LC3.

Molecular oral microbiology·2015
Same author

Lymphoid susceptibility to the Aggregatibacter actinomycetemcomitans cytolethal distending toxin is dependent upon baseline levels of the signaling lipid, phosphatidylinositol-3,4,5-triphosphate.

Molecular oral microbiology·2015
Same author

Radiation hazards of primary cosmic particles.

AFMDC TR. Air Force Missile Development Center (U.S.)·2014
Same author

Allograft outcomes in outbred mice.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2013
Same author

Evaluation of the Maquet Neonatal and Pediatric Quadrox I with an integrated arterial line filter during cardiopulmonary bypass.

Perfusion·2012

Area of Science:

  • Biophysics
  • Cytogenetics
  • Optical Engineering

Background:

  • Flow cytometry is a powerful technique for chromosome analysis.
  • Developing advanced instrumentation is crucial for improving resolution and efficiency in cytogenetic studies.
  • Existing instruments often rely on water-cooled lasers, presenting potential limitations.

Purpose of the Study:

  • To construct and evaluate an experimental flow cytometer for high-resolution chromosome analysis.
  • To assess the performance of multilaser excitation using helium-cadmium (HeCd) lasers.
  • To compare the resolution of the experimental instrument with a standard flow cytometer.

Main Methods:

  • Construction of an experimental flow cytometer featuring a quartz flow cell and a 1.22 NA lens.

Related Experiment Videos

  • Utilized crossed cylindrical quartz lenses for multilaser excitation.
  • Employed two helium-cadmium (HeCd) lasers (16 mW at 442 nm and 35 mW at 325 nm) for specific fluorophore excitation.
  • Obtained bivariate flow karyotypes from normal human male chromosomes and the Daudi cell line.
  • Compared results with a standard instrument using dual water-cooled lasers.

    • Main Results:

    • The experimental flow cytometer achieved resolution comparable to a standard instrument.
    • Bivariate flow karyotypes were successfully generated for human chromosomes and the Daudi cell line.
    • Reduced laser power (10 mW HeCd laser) still yielded good, albeit slightly decreased, resolution.

    Conclusions:

    • The experimental flow cytometer design is effective for high-resolution bivariate flow karyotyping.
    • Multilaser excitation with HeCd lasers provides a viable alternative for chromosome analysis.
    • The instrument demonstrates potential for robust cytogenetic applications with comparable performance to established systems.