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Expression of the c-kit mRNA in human aortic endothelial cells
T Miyamoto1, Y Sasaguri, K Sugama
1Department of Pathology, Kurume University School of Medicine, Japan.
Biochemistry and Molecular Biology International
|October 1, 1994
Abstract:
We investigated the expression of the c-kit gene in human aortic endothelial cells. RT-PCR and Northern blot analyses showed that a human aortic endothelial cell line expressed mRNA specific for the c-kit gene. Furthermore, the addition of stem cell factor stimulated the cells to increase the amount of c-kit mRNA transcribed in response to its ligand. In situ hybridization also revealed that endothelial cells in vivo express the c-kit gene.