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Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay
1Developmental Biology Research Centre, King's College, University of London, UK.
Molecular Biotechnology
|June 1, 1994
Abstract:
A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease protection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.
