Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein (western) blotting

D Egger1, K Bienz

  • 1Institute for Medical Microbiology, University of Basel, Switzerland.

Molecular Biotechnology
|June 1, 1994
PubMed
Summary
This summary is machine-generated.

This review details protein (Western) blotting techniques for sensitive protein analysis. It covers separation, transfer, detection, and interaction characterization, emphasizing adaptable methods for specific research questions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ångstrom-Depth Resolution with Chemical Specificity at the Liquid-Vapor Interface.

Physical review letters·2023
Same author

Analysis of the lymphoid system in NOD/LtSz-scid IL2Rγ(null) mice engrafted with purified cord blood derived stem cells.

Journal of stem cells & regenerative medicine·2014
Same author

Electron microscopic silver enhancement for double labeling with antibodies raised in the same species.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Colloidal gold staining and immunoprobing on the same Western blot.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Electron microscopic silver enhancement for double labeling with antibodies raised in the same species.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Colloidal gold staining and immunoprobing on the same Western blot.

Methods in molecular biology (Clifton, N.J.)·2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Protein analysis is crucial for understanding biological processes.
  • Western blotting is a widely used technique for protein detection and characterization.

Purpose of the Study:

  • To provide a comprehensive review of protein (Western) blotting procedures.
  • To highlight methods for sensitive protein detection and analysis.
  • To discuss adaptations for studying protein-nucleic acid and protein-protein interactions.

Main Methods:

  • Electrophoretic separation of proteins.
  • Transfer of proteins to membranes.
  • Immunological and non-immunological detection systems.
  • Characterization of molecular interactions.

Related Experiment Videos

Main Results:

  • Detailed description of each step in the Western blotting workflow.
  • Emphasis on achieving high sensitivity in protein detection.
  • Discussion of variations for tailored experimental approaches.

Conclusions:

  • Western blotting is a versatile technique adaptable to diverse research needs.
  • Optimized methods enhance sensitivity for accurate protein analysis.
  • The review provides a practical guide for researchers using Western blotting.