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Related Experiment Videos

B-cell activation by helper T-cell membranes

M R Kehry1, P D Hodgkin

  • 1Department of Inflammatory Diseases, Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT 06877, USA.

Critical Reviews in Immunology
|January 1, 1994
PubMed
Summary

Activated helper T (Th) cells stimulate B cell proliferation and antibody production through cell contact and soluble signals. CD40 on B cells and its ligand on Th cells are crucial for this interaction, enabling new antibody generation.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Resting B cells require signals from activated helper T (Th) cells for proliferation and differentiation into antibody-producing cells.
  • Understanding B cell activation involves studying molecular interactions and signaling pathways mediated by Th cells.

Purpose of the Study:

  • To investigate the roles of cell contact and soluble factors from Th cells in B cell activation.
  • To characterize the molecular mechanisms, including the CD40-CD40 ligand interaction, governing B cell responses.
  • To explore methods for generating novel antibodies in vitro using recombinant molecules.

Main Methods:

  • Utilizing purified plasma membranes from activated Th cell clones and recombinant lymphokines to study B cell responses.
  • Investigating the effects of cell contact versus soluble signals on B cell proliferation and differentiation.

Related Experiment Videos

  • Analyzing the requirement for CD40 crosslinking on B cells for signal delivery.

    • Main Results:

    • B cell-Th cell contact alone can provide the proliferative signal, independent of lymphokines.
    • The CD40 receptor on B cells and its ligand on Th cells are critical for contact-mediated signaling.
    • Lymphokines are essential for B cell differentiation and isotype switching when delivered during DNA replication.
    • High CD40 crosslinking on B cells is necessary for effective contact signal delivery.

    Conclusions:

    • B cell activation is a complex process involving both contact-dependent and soluble signals from Th cells.
    • The CD40-CD40 ligand pathway is a key mediator of T cell-B cell interactions.
    • Recombinant molecules and membrane fractions offer potential for in vitro antibody production.