Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High resolution structures of HIV-1 RT from four RT-inhibitor complexes

J Ren1, R Esnouf, E Garman

  • 1Laboratory of Molecular Biophysics, Oxford, UK.

Nature Structural Biology
|April 1, 1995
PubMed
Summary

Researchers determined the structures of HIV-1 reverse transcriptase (RT) complexes with non-nucleoside inhibitors. These structures reveal a common binding mode involving hydrophobic interactions and induced shape complementarity for diverse compounds.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extreme plate boundary localization promotes shallow earthquake slip at the Japan Trench.

Science (New York, N.Y.)·2025
Same author

Vaccine microarray patch self-administration: A preliminary study in adults 50 years of age and over.

Vaccine·2025
Same author

Silicosis initially presenting with empyema.

Occupational medicine (Oxford, England)·2024
Same author

Vaccine microarray patch self-administration: An innovative approach to improve pandemic and routine vaccination rates.

Vaccine·2023
Same author

Repeatable glucocorticoid expression is associated with behavioural syndromes in males but not females in a wild primate.

Royal Society open science·2019
Same author

Protocol for a Randomised controlled trial to Evaluate the effectiveness and cost benefit of prescribing high dose FLuoride toothpaste in preventing and treating dEntal Caries in high-risk older adulTs (reflect trial).

BMC oral health·2019

Area of Science:

  • Structural Biology
  • Virology
  • Medicinal Chemistry

Background:

  • HIV-1 reverse transcriptase (RT) is a key enzyme in the viral replication cycle.
  • Non-nucleoside inhibitors are crucial for HIV-1 treatment, but their binding mechanisms require detailed structural understanding.

Purpose of the Study:

  • To elucidate the structural basis of non-nucleoside inhibitor binding to HIV-1 RT.
  • To characterize the interactions between diverse inhibitors and the enzyme active site.

Main Methods:

  • X-ray crystallography was used to determine the high-resolution structures of four HIV-1 RT complexes.
  • Detailed structural analysis of the enzyme-inhibitor interfaces.

Main Results:

  • Three high-resolution structures of HIV-1 RT complexes with non-nucleoside inhibitors were determined.

Related Experiment Videos

  • The RT-nevirapine complex structure was resolved to 2.2 Å with an R-factor of 0.186.
  • A common binding mode was identified for chemically diverse inhibitors, characterized by hydrophobic interactions and induced fit.

    • Conclusions:

    • Non-nucleoside inhibitors bind to HIV-1 RT through a conserved mechanism involving enzyme and inhibitor conformational changes.
    • Induced shape complementarity driven by hydrophobic interactions is key to inhibitor binding.
    • These findings provide a structural basis for the design of novel HIV-1 RT inhibitors.