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Improved microbiological assay for folic acid based on microtiter plating with Streptococcus faecalis
J Das Sarma1, C Duttagupta, E Ali
1Indian Statistical Institute, Calcutta.
Journal of AOAC International
|September 1, 1995
Summary
This study introduces a faster, more sensitive microbiological assay for folic acid using 96-well plates. The improved method simplifies procedures and reduces analysis time for accurate folic acid measurement.
Area of Science:
- Microbiology
- Analytical Chemistry
- Biochemistry
Background:
- Conventional microbiological assays for folic acid are often tedious and time-consuming.
- There is a need for more efficient and sensitive methods for folic acid determination.
Purpose of the Study:
- To develop an improved, high-throughput microbiological assay for folic acid.
- To enhance the sensitivity and reduce the time required for folic acid analysis.
Main Methods:
- Utilized 96-well microtiter plates for a streamlined microbiological assay.
- Reduced culture volumes by 33-fold and simplified pipetting procedures.
- Stored bacterial suspension aliquots in saline at 4°C to minimize day-to-day variability.
Main Results:
- Achieved a 10-fold increase in sensitivity, reaching 0.1 ng/mL.
- Reduced assay time to 14 hours.
- Demonstrated high analytical recovery (98-104%) and low variability (<11%).
Conclusions:
- The developed microtiter plate assay significantly decreases the tedium of traditional methods.
- The assay is accurate, selective, and suitable for direct folic acid measurement in multivitamin formulations.
- This method offers a more efficient approach to folic acid quantification.