[In vitro activity of disinfectants against bacteria isolated in water for hemodialysis]

The bactericidal and sporicidal activities of six disinfectants (Acétoper, Cidex, Phagoseptyl, Pyosynthène, Bétadine, Sterlane) were studied against 21 bacterial strains recovered from hemodialysis machines, and against two bacterial spores. AFNOR methods were used (NF T. 72-151, NF T. 72-171 et NF T. 72-231). Only Acétoper presented a bactericidal and sporicidal activity. A disparity in bactericidal concentrations was observed among Gram negative bacteria. Phenolic compound (Phagoseptyl) was not influenced by interfering substances. pH have a clear effect upon Cidex and Bétadine activities. Statistical analysis (variance and multiple comparisons test of Ryan-Einot) showed the significant role of the type of bacteria and the nature of disinfectants. These compounds were classified according to their increasing activity as following: Cidex, Sterlane, Phagoseptyl, Bétadine, Pyosynthène, Acétoper.