Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Postseptational chromosome partitioning in bacteria

M E Sharpe1, J Errington

  • 1Sir William Dunn School of Pathology, University of Oxford, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|September 12, 1995
PubMed
Summary

The SpoIIIE protein aids chromosome partitioning in Bacillus subtilis vegetative cells, especially when cell division is disrupted. It helps recover trapped chromosomes and enhances antibiotic resistance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HEIDI: an experiment-management platform enabling high-throughput fragment and compound screening.

Acta crystallographica. Section D, Structural biology·2024
Same author

Population pharmacokinetics of carboplatin, etoposide and melphalan in children: a re-evaluation of paediatric dosing formulas for carboplatin in patients with normal or mild impairment of renal function.

British journal of clinical pharmacology·2018
Same author

Pooling of genital swabs for detection by PCR of Taylorella equigenitalis, the cause of contagious equine metritis.

Equine veterinary journal·2018
Same author

Increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in England and Wales since 2001.

Veterinary microbiology·2012
Same author

Prevalence of Coxiella burnetii in livestock abortion material using PCR.

The Veterinary record·2011
Same author

Development and validation of RT-PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gammacoronaviruses within clinical samples.

Transboundary and emerging diseases·2011

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The spoIIIE gene is crucial for chromosome partitioning during bacterial sporulation.
  • Its presence in vegetative cells and related genes in non-sporulating bacteria suggest a broader role.
  • Previous studies indicated spoIIIE's importance in sporulation but its vegetative function was unclear.

Purpose of the Study:

  • To investigate the function of SpoIIIE protein in vegetative Bacillus subtilis cells.
  • To determine if SpoIIIE plays a role in chromosome partitioning under perturbed conditions.
  • To explore SpoIIIE's contribution to antibiotic resistance.

Main Methods:

  • Genetic analysis of Bacillus subtilis mutants.
  • Observation of chromosome partitioning during vegetative growth under altered septation and nucleoid partitioning.

Related Experiment Videos

  • Assessment of antibiotic resistance in wild-type and mutant strains.

    • Main Results:

    • SpoIIIE protein is essential for optimal chromosome partitioning in vegetative cells when cell division coordination is disrupted.
    • SpoIIIE facilitates the recovery of chromosomes trapped by the closing septum.
    • SpoIIIE enhances resistance to antibiotics that target DNA metabolism.

    Conclusions:

    • SpoIIIE has a general function in bacterial chromosome partitioning, extending beyond sporulation.
    • The protein likely translocates chromosomes to ensure proper segregation during cell division.
    • SpoIIIE plays a role in maintaining genomic integrity and cellular defense mechanisms.