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Related Experiment Video

Updated: Jul 23, 2026

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

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CD27/CD70 interaction directly drives B cell IgG and IgM synthesis

K Agematsu¹, T Kobata, F C Yang

¹Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.

European Journal of Immunology

|October 1, 1995

Summary

The CD27-CD70 interaction significantly boosts B cell antibody production, leading to increased IgG and IgM secretion. This finding highlights a key pathway in B cell differentiation and antibody synthesis.

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Area of Science:

Immunology
Cell Biology

Background:

CD27 is a T cell activation marker found on most peripheral T cells and a subset of B cells.
CD70, the ligand for CD27, is expressed on activated T and B cells, suggesting a role in cell-cell communication.

Purpose of the Study:

To investigate the role of the CD27-CD70 interaction in B cell immunoglobulin synthesis.
To analyze how CD70 affects B cell growth and antibody production.

Main Methods:

Utilized a CD70 transfectant to study B cell immunoglobulin synthesis.
Employed flow cytometry to analyze CD27 and CD70 expression on activated B cells.
Assessed B cell proliferation and antibody production in response to CD70 stimulation.

Main Results:

CD27 and CD70 expression increased on B cells activated with Staphylococcus aureus Cowan strain (SAC) plus IL-2.
CD70 stimulation mildly enhanced B cell proliferation, which was blocked by anti-CD70 antibodies.
CD70 transfectant significantly increased IgG and IgM production by purified B cells, an effect blocked by anti-CD70 or anti-CD27 antibodies.

Conclusions:

The interaction between CD27 and its ligand CD70 is crucial for B cell growth.
CD27-CD70 ligation plays a significant role in B cell differentiation and the production of IgG and IgM antibodies.