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Mouse lymphotoxin-beta receptor. Molecular genetics, ligand binding, and expression
W R Force1, B N Walter, C Hession
1Division of Biomedical Sciences, University of California, Riverside 92521, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1995
Summary
Researchers identified the mouse Lymphotoxin beta receptor (LT beta R), crucial for immune responses and lymph node development. This discovery aids understanding of the LT beta R cytokine-receptor system in immunity.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Lymphotoxin (LT)-alpha beta heterotrimer is a T cell-expressed ligand binding the LT beta receptor (LT beta R).
- LT beta R is vital for lymph node development and immune reactions.
Purpose of the Study:
- To isolate and characterize the mouse LT beta receptor (LT beta R).
- To investigate the role of the LT beta R cytokine-receptor system in immune function.
Main Methods:
- Isolated mouse cDNA encoding LT beta R.
- Constructed an extracellular domain chimera to test receptor binding.
- Analyzed gene structure, mapping, and mRNA expression.
Main Results:
- Identified a 415 amino acid mouse LT beta R with 76% identity to human LT beta R.
- Demonstrated receptor function via binding assays.
- Mapped the Ltbr gene to mouse chromosome 6 and analyzed its expression patterns.
Conclusions:
- The characterization of mouse LT beta R provides a tool for studying its role in immune development and function.
- This research facilitates further investigation into the LT beta R cytokine-receptor system.