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Related Experiment Videos

Interleukin-10 in human milk

R Garofalo1, S Chheda, F Mei

  • 1University of Texas Medical Branch, Department of Pediatrics, Galveston 77555-0369, USA.

Pediatric Research
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

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Interleukin-10 (IL-10) is present in human milk, potentially offering anti-inflammatory benefits to infants. This bioactive cytokine was found in both aqueous and lipid fractions, suggesting protective roles in the infant

Area of Science:

  • Immunology
  • Neonatal Nutrition
  • Lactation Biology

Background:

  • Human milk contains numerous bioactive components crucial for infant development and immunity.
  • Interleukin-10 (IL-10) is a key cytokine with known immunomodulatory and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the presence, characteristics, and bioactivity of IL-10 in human milk during early lactation.
  • To explore the potential source and functional implications of IL-10 in human milk for the infant.

Main Methods:

  • Quantification of immunoreactive IL-10 in aqueous and lipid milk fractions using immunoassays.
  • Gel filtration chromatography to determine IL-10 molecular weight.
  • Bioactivity assays using human blood lymphocytes and [3H]thymidine uptake.

Related Experiment Videos

  • Detection of IL-10 mRNA and protein in cultured mammary epithelial cells and milk leukocytes.

    • Main Results:

    • Immunoreactive IL-10 was detected in human milk, with concentrations ranging from 66 to 9301 pg/mL.
    • IL-10 was found in both aqueous and lipid fractions and in a high molecular weight complex.
    • Human milk IL-10 demonstrated bioactivity by inhibiting lymphocyte proliferation, an effect partially reversed by anti-IL-10 antibodies.
    • IL-10 mRNA was present in mammary epithelial cells, but protein production was not confirmed; milk leukocytes contained some IL-10 but were not identified as the primary source.

    Conclusions:

    • Bioactive IL-10 is present in human milk, likely in a protected form.
    • Human milk IL-10 may exert immunomodulatory and anti-inflammatory effects on the infant's gastrointestinal tract.
    • Further research is warranted to fully elucidate the origin and specific functions of IL-10 in neonatal immunity via human milk.