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Related Experiment Videos

Capturing genes encoding membrane and secreted proteins important for mouse development

W C Skarnes1, J E Moss, S M Hurtley

  • 1Biotechnology and Biological Sciences Research Council, Centre for Genome Research, University of Edinburgh, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|July 3, 1995
PubMed
Summary
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Genome biology·2002

Researchers developed a gene trap method to identify mutations in secreted and membrane proteins. Gene disruptions in LAR and PTP kappa phosphatases did not affect mouse embryonic development.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Identifying mutations in secreted and membrane proteins is crucial for understanding cellular processes.
  • Gene trapping is a powerful technique for discovering gene functions through insertional mutagenesis.

Purpose of the Study:

  • To develop and apply a novel gene trap strategy for prescreening mouse embryonic stem cells.
  • To identify insertional mutations in genes encoding secreted and membrane-spanning proteins.
  • To investigate the developmental roles of protein-tyrosine phosphatases LAR and PTP kappa.

Main Methods:

  • Development of a "secretory trap" utilizing N-terminal signal sequence capture for beta-galactosidase fusion protein generation.
  • Prescreening of mouse embryonic stem cells for insertional mutations.

Related Experiment Videos

  • Analysis of homozygous mice with gene disruptions in LAR and PTP kappa.

    • Main Results:

    • Successful identification of insertional mutations in genes including cadherin, netrin, sek receptor tyrosine kinase, LAR, and PTP kappa.
    • Homozygous disruption of LAR and PTP kappa genes was confirmed.
    • Neither LAR nor PTP kappa disruption resulted in essential defects in normal embryonic development.

    Conclusions:

    • The secretory trap is an effective method for identifying mutations in target genes.
    • LAR and PTP kappa are not essential for embryonic development in mice, suggesting functional redundancy or alternative pathways.