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Related Experiment Videos

Inositol phosphate structural requisites for Ca2+ influx

S DeLisle1, G W Mayr, M J Welsh

  • 1Veterans Affairs Medical Center, Iowa City, Iowa, USA.

The American Journal of Physiology
|June 1, 1995
PubMed
Summary
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Inositol phosphates (InsP) trigger calcium (Ca2+) influx by depleting intracellular stores, acting via the inositol 1,4,5-trisphosphate receptor. High InsP concentrations are needed to override store refilling mechanisms.

Area of Science:

  • Cellular Biology
  • Molecular Physiology
  • Calcium Signaling

Background:

  • Inositol phosphates (InsP) are crucial signaling molecules involved in various cellular processes.
  • Understanding the precise mechanisms by which InsP regulate calcium (Ca2+) homeostasis is vital for cell function.

Purpose of the Study:

  • To elucidate the mechanism by which inositol phosphates (InsP) induce calcium (Ca2+) influx.
  • To identify specific InsP structures responsible for Ca2+ influx and their cellular targets.

Main Methods:

  • Injection of 37 purified inositol phosphate compounds (49 positional isomers) into Xenopus oocytes.
  • Measurement of intracellular Ca2+ release and Ca2+ influx in response to InsP stimulation.

Main Results:

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  • Eight specific InsP isomers stimulated Ca2+ influx, correlating with high potency for releasing intracellular Ca2+.
  • High concentrations of InsP were required for Ca2+ influx, exceeding those needed for intracellular Ca2+ release.
  • InsP-induced Ca2+ influx inhibited further intracellular Ca2+ release and was not accompanied by additional release during influx.

Conclusions:

  • The findings support the capacitative Ca2+ entry hypothesis, where InsP stimulate Ca2+ influx by depleting intracellular Ca2+ stores.
  • Sufficiently high InsP concentrations are necessary to overcome store refilling mechanisms and induce Ca2+ influx.