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PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat
J M Rodríguez1, L M Cintas, P Casaus
1Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Applied and Environmental Microbiology
|July 1, 1995
Abstract:
The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results.