Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phase-sensitive two-dimensional experiment on living animals

B Gillet1, B T Doan

  • 1Laboratoire de RMN biologique, ICSN-CNRS, Gif Sur Yvette, France.

Journal of Magnetic Resonance. Series B
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Confirmed validation of an innovative PCR-assay without DNA extraction for multiplex diagnosis of factor V Leiden and prothrombin gene variants.

Thrombosis research·2019
Same author

Scapular kinematics during scaption in competitive swimmers.

European journal of sport science·2018
Same author

Scapulohumeral rhythm in young tennis players.

Computer methods in biomechanics and biomedical engineering·2017
Same author

Dramatic Increase in Incidence of Ulcerative Colitis and Crohn's Disease (1988-2011): A Population-Based Study of French Adolescents.

The American journal of gastroenterology·2017
Same author

New parameters on the hematology analyzer XN-10 (SysmexTM) allow to distinguish childhood bacterial and viral infections.

International journal of laboratory hematology·2016
Same author

Scapulothoracic kinematics during scaption after one year of tennis practice in elite girl players.

Computer methods in biomechanics and biomedical engineering·2015
Same journal

NMR pulse schemes for the sequential assignment of arginine side-chain H epsilon protons.

Journal of magnetic resonance. Series B·1996
Same journal

In vivo imaging of nitroxide-free-radical clearance in the rat, using radiofrequency longitudinally detected ESR imaging.

Journal of magnetic resonance. Series B·1996
Same journal

Extracting quantitative information from two- and three-dimensional NOE spectra measured with short recycle delays.

Journal of magnetic resonance. Series B·1996
Same journal

HCN, a triple-resonance NMR technique for selective observation of histidine and tryptophan side chains in 13C/15N-labeled proteins.

Journal of magnetic resonance. Series B·1996
Same journal

A three-dimensional NMR imaging scheme utilizing doubly resonant gradient coils.

Journal of magnetic resonance. Series B·1996
Same journal

Observing N-acetyl aspartate via both its N-acetyl and its strongly coupled aspartate groups in in vivo proton magnetic resonance spectroscopy.

Journal of magnetic resonance. Series B·1996
See all related articles

This study presents the first in vivo 1H-1H Total Correlation Spectroscopy (TOCSY) spectrum from mouse muscle. TOCSY offers superior molecular detail and sensitivity for studying fatty acids compared to COSY.

Area of Science:

  • Biophysics
  • Molecular Spectroscopy
  • In Vivo Imaging

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is crucial for molecular analysis.
  • Distinguishing molecular structures in complex biological tissues remains challenging.
  • Previous methods like COSY have limitations in spectral resolution and sensitivity.

Purpose of the Study:

  • To report the first in vivo 1H-1H Total Correlation Spectroscopy (TOCSY) spectrum acquired from the hind-leg muscles of an intact mouse.
  • To evaluate the advantages of TOCSY over COSY for molecular characterization in biological systems.
  • To assess TOCSY's utility for analyzing long fatty-acid chains.

Main Methods:

  • Acquisition of a 1H-1H phased spectrum using TOCSY.
  • In vivo spectroscopy performed on the hind-leg muscles of an intact mouse.

Related Experiment Videos

  • Comparison of TOCSY and COSY (Correlation Spectroscopy) for spectral analysis and sensitivity.

    • Main Results:

    • Successfully obtained the first in vivo 1H-1H phased spectrum from mouse hind-leg muscles.
    • Demonstrated that TOCSY can trace complete molecular spin systems, providing more detailed molecular graphs than COSY.
    • Showcased TOCSY's enhanced suitability for studying long fatty-acid chains.
    • Observed that TOCSY spectra have all peaks in phase and are less sensitive to digitalization issues than COSY spectra.
    • Quantified sensitivity improvements by comparing cross-correlation peak volumes in COSY and TOCSY.

    Conclusions:

    • In vivo TOCSY is a powerful technique for detailed molecular analysis in biological tissues.
    • TOCSY provides significant advantages over COSY in terms of spectral completeness, sensitivity, and suitability for analyzing complex molecules like fatty acids.
    • This advancement opens new avenues for in vivo metabolic studies and molecular profiling.