Related Experiment Videos
Transglutaminase activity in enterocytes isolated from pig jejunum
H M'Rabet-Touil1, F Blachier, N Hellio
1Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Molecular and Cellular Biochemistry
|May 10, 1995
Summary
Pig enterocytes use extracellular polyamines, incorporating them into proteins via transglutaminase. This enzyme activity is crucial for intestinal mucosa integrity and function.
Area of Science:
- Gastroenterology
- Cell Biology
- Biochemistry
Background:
- Polyamines are vital for intestinal mucosa integrity, turnover, and growth.
- Transglutaminase may mediate polyamine incorporation into cellular proteins.
Purpose of the Study:
- To measure transglutaminase activity in pig jejunum enterocytes.
- To investigate polyamine incorporation and metabolism in isolated enterocytes.
Main Methods:
- Isolation of villus enterocytes from pig jejunum.
- Measurement of transglutaminase activity in cell homogenates.
- Assessment of polyamine incorporation into TCA precipitable material.
Main Results:
- Enterocytes incorporate exogenous polyamines (putrescine, spermidine, spermine) into proteins.
- Transglutaminase activity was confirmed, dependent on time and substrate, and inhibited by glycine methyl ester.
- Enterocytes exhibit limited de novo polyamine synthesis, indicated by low ornithine decarboxylase activity.
Conclusions:
- The primary source of polyamines for pig enterocytes is extracellular.
- Exogenous polyamines serve as substrates for enterocyte transglutaminase, supporting mucosal integrity.