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Modeling and optimising dextrose fermentation using a fluorosensor

S Sundaram1, R Padmanabhan, S Gowrishankar

  • 1Department of Chemical Engineering, Regional Engineering College, Tiruchirappalli, India.

Biomedical Sciences Instrumentation
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

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The optimal yeast seeding concentration for dextrose fermentation is 30 percent, maximizing biomass growth and final concentration. This finding aids in optimizing fermentation processes for increased yield.

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Dextrose fermentation is a key bioprocess for producing valuable compounds.
  • Optimizing yeast seeding concentration is crucial for maximizing fermentation efficiency and yield.

Purpose of the Study:

  • To determine the optimal yeast seeding concentration for dextrose fermentation.
  • To monitor fermentation progress using NADH fluorescence.
  • To establish a kinetic model for the fermentation process.

Main Methods:

  • Fermentation of dextrose in a Tokyo Rikakikai Fermentor at 32°C.
  • Utilized seven different yeast seeding concentrations.
  • Monitored reaction progress via NADH fluorescence using a Dr. Ingold fluorosensor (360 nm excitation, 450 nm emission).

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Main Results:

  • The optimal yeast seeding concentration was identified as 30 percent.
  • This concentration resulted in maximum biomass growth rate and final biomass concentration.
  • Fluorescent voltage versus time data accurately fitted a first-order kinetic model with <1% error.

Conclusions:

  • A 30% yeast seeding concentration is optimal for dextrose fermentation under the tested conditions.
  • NADH fluorescence provides a reliable method for real-time monitoring of fermentation progress.
  • The fermentation follows predictable first-order kinetics, enabling process modeling and optimization.