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Updated: Jul 12, 2026
Measuring Local Anaphylaxis in Mice
Published on: October 14, 2014
Micro-assay to measure the allergenicity of a Kunitz-type soybean trypsin inhibitor toward Balb/c mice by using
R Yamanishi1, K Kondo, H Tsuji
1Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Abstract:
The allergenicity of a Kunitz-type soybean trypsin inhibitor (KSTI) was investigated by a micro-assay of beta-N-acetylhexosaminidase released from RBL-2H3 cells primed with the anti-KSTI serum. KSTI stimulated the release of beta-N-acetylhexosaminidase from RBL-2H3 cells primed with the antiserum. The response of RBL-2H3 cells to the reaginic activity of the mouse anti-KSTI serum correlates fairly well with that by the passive cutaneous anaphylaxis (PCA) test, the sensitivity of both assays appearing to be similar. These results suggest that measuring the beta-N-acetylhexosaminidase released from RBL-2H3 is a convenient way for studying the allergen or the reaginic activity of a murine serum in place of the PCA test.
