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A molecular marker based linkage map of Vitis
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell Unversity, Geneva 14456, USA.
Genome
|August 1, 1995
Summary
Researchers developed genetic linkage maps for grapevines using RAPD, RFLP, and isozyme markers. These detailed maps of
Area of Science:
- Plant Genetics
- Genomics
- Vitis Vinifera Research
Background:
- Genetic mapping is crucial for understanding crop improvement in Vitis species.
- Developing high-density genetic maps aids in identifying genes controlling important traits.
Purpose of the Study:
- To construct high-resolution genetic linkage maps for two Vitis genotypes, 'Cayuga White' and 'Aurore'.
- To provide a genomic framework for future quantitative trait locus (QTL) analysis and map-based gene cloning in grapes.
Main Methods:
- Utilized an interspecific hybrid population of 60 grape seedlings from 'Cayuga White' X 'Aurore'.
- Employed a double-pseudotestcross strategy with 422 Random Amplified Polymorphic DNA (RAPD) markers, alongside 16 Restriction Fragment Length Polymorphism (RFLP) and isozyme markers.
- Constructed individual linkage maps for each parent, identifying homologous groups.
Main Results:
- Developed integrated genetic maps with an average marker distance of 6.1 cM.
- 'Cayuga White' map: 214 markers, 1196 cM, 20 linkage groups.
- 'Aurore' map: 225 markers, 1477 cM, 22 linkage groups.
- Integrated map comprised 19 linkage groups, indicating homology between parental groups.
Conclusions:
- The generated genetic maps offer comprehensive genome coverage for Vitis.
- These maps are valuable resources for identifying genes related to desirable traits and facilitating marker-assisted breeding in grapevines.