Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA structure influences sequence specific cleavage by bleomycin

K P Nightingale1, K R Fox

  • 1Department of Physiology & Pharmacology, University of Southampton, UK.

Nucleic Acids Research
|June 11, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized evaluation of an online single-session intervention for minority stress in LGBTQ+ adolescents.

Internet interventions·2023
Same author

Psychosis as a risk factor for suicidal thoughts and behaviors: a meta-analysis of longitudinal studies.

Psychological medicine·2017
Same author

The Blood Donation Ambivalence Survey: measuring conflicting attitudes about giving blood.

Transfusion medicine (Oxford, England)·2017
Same author

Perceptions of success of a local UK public health collaborative†.

Health promotion international·2017
Same author

Biological risk factors for suicidal behaviors: a meta-analysis.

Translational psychiatry·2016
Same author

Letter to the Editor: Suicide as a complex classification problem: machine learning and related techniques can advance suicide prediction - a reply to Roaldset (2016).

Psychological medicine·2016
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Iron(II)-bleomycin DNA cleavage shows sequence specificity, efficiently cutting (AT)n repeats at ApT sites. This DNA cleavage is structure-dependent, with strong binding requiring guanine.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Medicinal Chemistry

Background:

  • Bleomycin is a glycopeptide antibiotic known for its DNA-cleaving properties.
  • The precise sequence and structural determinants of bleomycin-induced DNA cleavage are not fully understood.
  • Understanding these mechanisms is crucial for developing targeted DNA-damaging agents.

Purpose of the Study:

  • To investigate the sequence specificity of DNA cleavage by iron(II)-bleomycin.
  • To elucidate the role of DNA secondary structure in bleomycin binding and cleavage.
  • To differentiate between DNA binding affinity and cleavage efficiency.

Main Methods:

  • Synthesis of various DNA sequences with specific repeating motifs (e.g., (AT)n, (TAA)n, (AC)n).
  • Analysis of DNA cleavage patterns induced by iron(II)-bleomycin using gel electrophoresis.

Related Experiment Videos

  • DNase I footprinting assays with cobalt-bleomycin to assess DNA binding affinity.

    • Main Results:

    • Iron(II)-bleomycin exhibits sequence preferences in DNA cleavage, with GC = GT > GA >> GG in mixed sequences.
    • Efficient cleavage of (AT)n sequences occurs exclusively at ApT sites, not TpA.
    • Blocks of three or more contiguous ATs are cleaved as efficiently as GpT, while isolated ApT sites show minimal cleavage.
    • Cleavage is specific for (AT)n, with minimal cleavage observed in (TAA)n, (TTA)n, (ATT)n, and (AAT)n sequences.
    • No cleavage occurs at ApC or CpA within (AC)n.(GT)n sequences, nor in An.Tn regions.
    • Cobalt-bleomycin binding shows no footprints in (AT)n regions, indicating weak binding despite efficient cleavage.

    Conclusions:

    • Bleomycin-mediated DNA cleavage is primarily determined by local DNA structure, particularly (AT)n repeats.
    • Strong binding of bleomycin to DNA appears to require the presence of a guanine residue.
    • The study distinguishes between DNA binding affinity and cleavage efficiency, suggesting distinct mechanisms govern each.