Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assembly-dependent expression of antigenic epitopes by beta 2-microglobulin(b)

R J Tatake1, R A Zeff

  • 1Department of Pathology, University of Connecticut Health Center, Farmington 06030.

Immunogenetics
|January 1, 1993
PubMed
Summary

Mouse beta 2-microglobulin (beta 2mb) exhibits new antigenic epitopes upon assembly with H-2 class I heavy chains. This reciprocal interaction influences both beta 2m antigenicity and MHC class I heavy chain presentation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disparate cleavage of poly-(ADP-ribose)-polymerase (PARP) and a synthetic tetrapeptide, DEVD, by apoptotic cells.

Apoptosis : an international journal on programmed cell death·2001
Same author

Receptor-mediated gene delivery to human peripheral blood mononuclear cells using anti-CD3 antibody coupled to polyethylenimine.

Gene therapy·2001
Same author

The immune response in halo nevi.

Journal of the American Academy of Dermatology·1997
Same author

Expression of HLA class I molecules assembled with structural variants of human beta2-microglobulin.

Immunogenetics·1997
Same author

Mutants of human beta2-microglobulin map an immunodominant epitope within the three-stranded beta-pleated sheet.

Transplantation·1997
Same author

beta 2-Microglobulin in transplantation.

Transplantation·1995

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Beta 2-microglobulin (beta 2m) is a crucial component of MHC class I molecules.
  • The assembly of beta 2m with MHC class I heavy chains is essential for proper immune function.
  • Specific epitopes on beta 2m may be conformation-dependent and influenced by heavy chain association.

Purpose of the Study:

  • To investigate the expression of specific beta 2-microglobulin (beta 2mb) epitopes.
  • To determine if these epitopes are dependent on assembly with H-2 class I heavy chains.
  • To elucidate the reciprocal influence of heavy chain association on beta 2m antigenicity.

Main Methods:

  • Flow cytometry analysis of cell lines with varying H-2 gene expression.
  • Use of beta 2mb-specific monoclonal antibodies (mAbs) S19.8 and clone 23.

Related Experiment Videos

  • Transfection of cell lines with wild-type H-2Kb gene.
  • In vitro assembly of H-2Kb heavy chains with beta 2mb.

    • Main Results:

    • Beta 2mb expression was observed in cells with mutant and wild-type H-2Db, but specific epitopes (detected by mAb S19.8 and clone 23) were absent in serum-containing conditions.
    • These beta 2mb epitopes were strongly expressed after transfection with wild-type H-2Kb, confirming dependence on heavy chain assembly.
    • In vitro assembly of H-2Kb with beta 2mb recovered these epitopes, while beta 2mb alone or in non-conformed states did not.

    Conclusions:

    • Specific beta 2mb epitopes are induced upon assembly with cognate H-2 class I heavy chains.
    • This assembly process reciprocally influences the antigenicity of beta 2m.
    • The findings highlight a coordinated antigenicity modulation between beta 2m and MHC class I heavy chains.