Related Experiment Video
Updated: Aug 10, 2026

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
Published on: June 29, 2016
[The carbohydrate-binding molecules of the surface membranes of immune system cells]
Abstract:
Data are presented on the use of biotinylated polyacrylamide attached carbohydrates as immunohistochemical probes. Their high specificity is shown, the optimal conditions of performing the immunocytochemical reaction are described. Using a panel of probes, oligosaccharide-binding molecules were revealed on the surface of lymphocytes from peripheral blood, lymph nodes and pleural effusions, and in the cytoplasm of phagocytic cells. Data on the expression of endogenous lectin-like molecules in different cells are presented.
Related Concept Videos
Fluid Mosaic Model
Membrane Carbohydrates
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Glycocalyx and its Functions
Components of...
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

