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Structure and expression of the mouse apolipoprotein C2 gene
M J Hoffer1, M M van Eck, L M Havekes
1MGC-Department of Human Genetics, Leiden University, The Netherlands.
Genomics
|July 1, 1993
Summary
Researchers identified mouse apolipoprotein C2 (Apoc2) gene variants, including a larger transcript potentially involved in alternative splicing. This finding offers new insights into Apoc2 gene regulation and function in mammals.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Apolipoprotein C2 (ApoC2) plays a crucial role in lipid metabolism.
- Understanding the genetic regulation of ApoC2 is essential for studying lipid disorders.
Purpose of the Study:
- To isolate and characterize cDNA clones of the mouse apolipoprotein C2 (Apoc2) gene.
- To investigate the exon-intron structure and transcriptional variants of the mouse Apoc2 gene.
Main Methods:
- cDNA library screening
- Sequence analysis
- Northern blotting
- Primer extension analysis
- RT-PCR analysis
Main Results:
- Three cDNA clones of the mouse Apoc2 gene were isolated, with sizes of 500 bp and 962 bp.
- The major liver transcript is 500 bp, encoded by four exons.
- Alternative splicing and an upstream transcription initiation site were detected, suggesting a larger, alternatively spliced transcript.
Conclusions:
- The mouse Apoc2 gene exhibits alternative splicing, producing transcripts of different sizes.
- These findings contribute to understanding the complex regulation of Apoc2 gene expression.