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Related Experiment Videos

CD48 is a low affinity ligand for human CD2

M S Sandrin1, E Mouhtouris, H A Vaughan

  • 1Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.

Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1993
PubMed
Summary
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The study reveals that CD48 binds to the CD2 molecule on human peripheral blood lymphocytes (PBL). This interaction is similar to CD2 binding to CD58 (LFA-3), suggesting CD48 is an alternative ligand for CD2.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Interactions

Background:

  • The CD2 molecule is a key receptor on T lymphocytes involved in cell adhesion and activation.
  • CD58 (LFA-3) is a known ligand for CD2, mediating T cell interactions with other cells.
  • The potential ligands and interaction partners of CD2 are of significant interest in understanding immune cell communication.

Purpose of the Study:

  • To investigate the interaction between human CD48 and the CD2 molecule.
  • To determine if CD48 can bind to human peripheral blood lymphocytes (PBL).
  • To characterize the binding site and affinity of the CD2-CD48 interaction.

Main Methods:

  • Transient transfection of COS cells with human CD48.
  • Assessing the binding of human PBL to transfected COS cells.

Related Experiment Videos

  • Inhibition studies using monoclonal antibodies (mAbs) against CD48 and CD2.
  • Binding assays with a fluorescently labeled, multimeric form of recombinant CD2 (rCD2).

    • Main Results:

    • COS cells transfected with CD48 demonstrated binding to human PBL, unlike mock or CD7-transfected cells.
    • PBL binding to CD48 transfectants was significantly inhibited by anti-CD48 or anti-CD2 mAbs.
    • Fluorescent rCD2 bound to CD48-transfected cells in a CD48-dependent manner.
    • Blocking studies indicated CD48 interacts with the T11(1) region of CD2, the same site that binds CD58 (LFA-3).
    • CD48 binds CD2 with approximately 100-fold lower affinity compared to CD58.

    Conclusions:

    • CD48 acts as a ligand for the CD2 molecule on human PBL.
    • CD48 and CD58 (LFA-3) are alternative ligands for CD2, potentially competing for binding.
    • The CD2-CD48 interaction occurs at the T11(1) binding site, with lower affinity than the CD2-CD58 interaction.